BANC is the first full female Brain-And-Nerve-Cord volumetric EM data set. It was generated by Wei-Chung Lee’s lab, and segmented by Zetta AI. The FlyWire team at Princeton is responsible for systematic proofreading, beginning with DNs and ANs.

To get access to the BANC, please ask Rachel to contact Wei or follow this link to request access through Flywire. Note a few restrictions:

  • We cannot publish reconstructions from the dataset until Wei and his lab do so, or until December 31, 2024, whichever comes first.
  • Anyone using the BANC data prior to the first publication from WCL also must agree to include WCL and WCL lab members that generated the dataset (Jasper Phelps and Minsu Kim) as authors on their publication.
  • To publish neurons from FlyWire/BANC, a member lab must submit a list by emailing flywire@princeton.edu or through a planned online submission system, and then must make that list available in the paper.

Please see the attached document for full terms of use.

📎 Terms of Use - Proofreading Female CNS Dataset_revised.pdf

Searching the BANC#

Currently, all of the ANs and DNs have been proofread. The synapse predictions are months away from completion. If one is looking to find their AN/DN of interest now, it is a bit of a needle in a haystack situation, but there are ways to increase throughput.

For ANs, it is helpful to go to the x cross section of the neck connective within the EM volume. From there, you can go to an area of the neck connective you think your neuron may traverse through (i.e. more lateral or more medial). For DNs, a higher throughput way to do it is to click around the primary neurite tracks in the BANC. Then, you can click through different neurons and examine their morphology in the brain and the VNC. By eye, you can screen your neurons and add an annotation (see small yellow dots in image) onto neurons you have checked already. Eventually, you should find your neuron! Make sure to write down the coordinates and the ID of your neuron of interest so you can find it again. If desired, you can upload cell identifications using the banc-bot on the Flywire Slack.