Generic useful oscilloscope settings#

  • trigger: type: edge, source: AC line, slope: rising, mode:auto, coupling: noise reject
  • all channels: probe gain = 1×
  • no external trigger

Whole-cell patch-clamp recordings#

  • 40×WI objective
  • variable output on A-M Systems Model 2400 amplifier to A-to-D board; using Model 2400 filter this output at 5kHz, gain=50×, probe gain set to low
  • Im output on on A-M Systems Model 2400 amplifier → to input channel 1 on LPF202A auxillary amplifier; using the LPF202A amplify this 100× (filter on “bypass”) → to oscilloscope channel 2 (“current”) and A-to-D board
  • ×10Vm output on A-M Systems Model 2400 amplifier → to input channel 2 on LPF202A auxillary amplifier; using the LPF202A amplify this 1× and low-pass filter at 2kHz → to oscilloscope channel 1 (“voltage”)
  • Oscilloscope channel 1 (“voltage”) set to DC coupled, 88mV/div vertical scale, 100ms/div horizontal scale; channel 2 (“current”) set to DC coupled, 2V/div
  • amplifier in Iclamp or Vclamp mode
  • electrode angle ~30°

Single-sensillum recordings#

  • 50× air objective
  • put the condenser diaphragm completely open, and switch from the polarizer filter to the heat-shielding filter
  • ×10Vm output on A-M Systems Model 2400 amplifier → to input channel 2 on LPF202A auxillary amplifier; using the LPF202A amplify this 20× and low-pass filter at 2kHz → to oscilloscope channel 1 (“voltage”) and A-to-D boardoscilloscope channel 1 set to AC coupled, 88mV/div vertical scale, 100ms/div horizontal scale all other oscilloscope channels off amplifier in I=0 mode
  • electrode angle ~35°
  • antenna is stabilized by two hooks: one coming from the top and right (this should be on a Sutter manipulator) and the other from the left and bottom (a coarse manipulator will work); both the hooks should come in horizontally for best stability.
  • The method for stabilizing the palp is a little bit different than that for the antennae. The palp sits on a coverslip and it is anchored from the top by a cleaning pipette. The cleaning pipette should come at ~30°. The recording pipette should also come in at a steeper angle than for the antennae. 45° should work fine but there does not seem to be any reason not to go as high as the air-objective will allow.