Wilson Lab Wiki

      • Iontophoresis
      • Pressurised ATP delivery
      • Neuron activation and inhibition
      • Behavioral Genetics Experiments
      • LED Rings
      • Controlling enclosure temperature and humidity
      • Notes on getting robust behavior
      • G4 Arenas: Set up notes
      • Balls for behavior
      • Overview: closed-loop virtual reality for flies
      • G3 Arenas Part 3: pattern and function guide
      • G3 Arenas Part 2: software and general usage
      • Dissection guide
      • FLIR + Spinnaker + SpinView
      • FicTrac Part 3: closed-loop setup
      • G3 Arenas Part 1: assembly
      • FicTrac Part 1: initial installation
      • FicTrac Part 2: general usage
      • Scalebars with connectome volumes
      • BANC
      • Registration using CMTK plugin in FIJI
      • neuprint-python
      • FANC
      • FlyWire
      • Searching for Genetic Lines
      • Tracing from confocal images
      • Flycircuit database
      • FAFB v14/CATMAID/walled garden
      • Color-depth MIP searches
      • WLU: Signal processing
      • Labeling with neurobiotin and biocytin
      • Notes on Saline
      • Applying Dispase
      • De-noising a rig
      • Making internal
      • Making saline
      • Electrophysiology for Drosophilists
      • Patching guide
      • Electrophysiology rig configuration
      • 3D Printing (in house)
      • Replacing halogen lamps on dissection scopes
      • Gas Flow (in 2p and main lab)
      • Laser Permit
      • Fly Incubators
      • Laser Cutter
      • Mass flow controller (MFC)
      • Dissection hooks
      • MP-225
      • Pico Pump
      • Filter cubes
      • Pipette puller
      • Molecular biology equipment
      • Water purifier
      • Telephone
      • Solenoid valves
      • Optomechanical components
      • Copier - scanner
      • Radio Shack power cords
      • Printers
      • Piezoelectric actuators
      • pH Meter
      • Odor delivery devices
      • Lab laptop
      • Data acquisition cards (DAQ)
      • Balances
      • Using the lab's AI skills
      • Illustrator Tips
      • Image analysis resources
      • O2
      • Learning coding, data science, & stats
      • Server
      • Software Installations
      • HMS IT
      • Data back-up
      • Manuscript submissions
      • Room scheduling
      • Loading dock
      • Posters
      • Temperature (in lab)
      • NeuroOps
      • Neurobio Dept photocopier
      • Parking at HMS
      • Lockers
      • Library (Countway)
      • Imaging core facilities
      • Fax
      • Custodial Services
      • Lab Website
      • Lab Calendars
      • Writing a Thesis in the Program in Neuroscience (PiN) – Harvard GSAS
      • Unexpected visitor protocol
      • "Quick Fix" Meetings
      • Lab Birthdays
      • TTX (tetrodotoxin) SOP/SDS
      • Annual safety (EHS) inspection
      • Staying connected (seminars etc.,)
      • Offboarding
      • Laser permit
      • Lab photos
      • Lab Away Dates
      • Giving a talk
      • Postdoc onboarding
      • Mental health
      • Contact numbers
      • Lunch Train
      • Hazardous Wastes
      • Green Lab Practices
      • Lab Gmail (flypokers)
      • Czardoms
      • Cleanliness
      • Safety
      • Lab meeting schedule
      • New Lab Member Guide
      • New Research Assistant Guide
      • SendCutSend
      • Personnel-funding assignments
      • Protolabs
      • Amazon.com Purchases
      • Paying for WellGenetics order
      • Supply database
      • Vendor database
      • UPS
      • Purchasing tools
      • Lab Supplies
      • Software - Gene Construction Kit
      • Ordering through HMS (NIH projects)
      • Ordering -- HHMI ProCard
      • Ordering -- HMS PCard
      • Ordering through HHMI
      • Office Supplies
      • USPS
      • Fly Food
      • FedEx (Federal Express)
      • Purchasing ethanol
      • Dropbox (HMS accounts)
      • Conferences
      • Address format for shipping
      • Purchasing chemicals/drugs
      • Appliances
      • Billing Codes
      • Tax Certificates
      • MedTech Gas Ordering
      • Benchling (storing biological sequences)
      • Fly Food Supplies
      • Pipettes
      • Dissection Adhesives
      • Forcep sharpening with Corte Instruments
      • Immuno reagents log
      • Paraformaldehyde
      • Needles/syringes
      • Molecular biology supplies
      • Halo Tag Ligands
      • Gas tanks
      • Fly Holders
      • Antibodies
      • Data Acquisition WLU 2024
      • 210922 WLU on 2P imaging
      • 2-photon microscope configurations and information
      • Notes on setting up and de-noising an imaging rig
      • Notes on known issues with Berg-2
      • Spectra-Physics Laser
      • Berg status
      • Berg-2 operation
      • Laser permit
      • Coherent Vision-S laser
      • Microscope components and alignment
      • ScanImage Setup (Download + Installation)
      • Berg-1 two-photon microscope
      • 2P photostimulation + imaging
      • ScanImage Operation (Bergamo-2P)
      • Two-photon analysis pipeline
      • 2P troubleshooting (hardware/software)
      • Maintenance of multiphoton lasers
      • PCR for confirming transgenes
      • Flystocks database
      • 240207 WLU: Making transgenic flies
      • Fluorescence in situ hybridization (FISH)
      • Fly Making Overview (BestGene, Twist)
      • Plasmid Generation
      • Planning fly work around time away from the lab
      • Driver line collections
      • Fly food
      • Fly stock maintenance
      • Split-Gal4 screening (crosses)
      • WellGenetics services
      • Ordering/importing flies
      • When your flies arrive
      • Fly stock crediting, distribution, and shipping
      • Mites
      • Adult fly body anatomy
      • Landing sites for transgenes
      • Learning to do fly crosses
      • Useful markers for fly pushing
      • Genetic tools for neuroanatomy
      • Dissections & immunostaining

    Laser Permit

    📎 Laser permit.pdf

    Backward Gas Flow (in 2p and main lab) Fly Incubators Forward