BestGene can do injection services quickly and return balanced flies in a pretty painless way.

Account#

Our lab has an account to help keep track of past orders.

Username: flypokers@gmail.com Password: wilsonlab

Overview of making a fly:#

• To generate flies with a transgene in a specific landing site, you need to first pick one they support: https://www.thebestgene.com/PhiC31InfoPage.do. Note: you can use other sites (but it might be odd given the large selection), but you will need to supply them with the fly and use additional services not mentioned below.
• Next, you will need to ship them the plasmids for doing the injection. It is easiest to ship a small amount and have them amplify it.
• To ship a small amount of DNA and have BestGene generate flies with your transgenes in a specific landing site, you would use:
  • Service I: PhiC31 Premium - Plan H plus balancing (ship balanced line)
    • This means the only flies you will get are balanced, there are other options which are faster but will require more work on your end (e.g. plan H). Make sure you understand what they’ll be sending you.
  • Service Z1: Regular plasmid DNA Midiprep. One midiprep is enough for 3 “Service Quantity”
    • This allows you to send a smaller amount of DNA they can use for multiple injections.
• Fill out their order form (link at the top of their page), and attach a Purchase Order for the amount of money the order will cost. Alternatively, you can add a credit card to the account and have them charge that (but be careful! this can get expensive and you don’t want to hit the limit).
• Note they will want you to tell them the restriction enzyme sites to use for their quality checks - you will want to check that they work ahead of time. For the pJFRC7 and pJFRC19 uploaded to Twist, it appears that NotI and Xba1 work well.
• Ship them the DNA and plasmids, and wait patiently for the flies to trickle in.

Other notes#

• Their purchasing page has a list of other services and pricing here.
• Also see the page on WellGenetics /fly-stocks-and-crosses/wellgenetics-services/

Spreadsheet of attP landing sites and their locations

📎 attP landing sites.xlsx