Bloomington (BDSC)#

  • Sign up for your own account (click here) and select “Add User to Existing Account”.
  • User Number (BUN): 866
  • Account name: Rachel Wilson
  • Use your own account when placing orders (not Rachel’s account).
  • Do NOT select DOA (dead on arrival) unless you are replacing stocks that were actually DOA.
  • Bloomington now accepts payments by credit card, use the HHMI Pro-card for this. You will be alerted a few days after the order that you must pay, and can log back in, navigate to “orders” and click “Pay Now”
  • All stocks at Bloomington (including stocks not yet on Flybase) are here.
  • Bloomington stock subtypes are all listed here, including RNAi lines distinct from the VDRC stocks.
  • Bloomington does not ship during the second half of December.

WellGenetics#

  • When sending flies to WellGenetics, you’ll need  get a Taiwan import permit from them and then attach it to your shipping package.

KDRC (Korea)#

  • URL: kdrc.kr
  • Email: flypokers@gmail.com
  • Password : flypokers_wilsonlab123
  • On checkout upload the LONPR (attached below)
  • As of 4/2025 stocks are free, and we just need to provide the FedEx billing code (in the account already)
  • Update the shipping address on checkout! It does not populate properly by default.

VDRC (Vienna)#

  • URL: http://stockcenter.vdrc.at
  • User ID: WilsonLab
  • Email: flypokers@gmail.com
  • Password : flypokers_wilsonlab123
  • DO NOT use the HHMI card! International charges are not allowed. If you want to use HHMI funds reach out to our current science ops contact (as of 10/2023 this is Cindy Truong).
  • You can pay using the HMS P-card; VDRC will need to know the last 4 digits of the SSN for the P-Card (4990)
  • importation requires a USDA shipping label and a USDA “letter of no jurisdiction - see below
  • VDRC does not ship during the second half of December

Janelia FlyBank (split-Gal4)#

Exelixis (Harvard)#

  • User name: Wilson Lab
  • Password: flypokers
  • pay using the P-card

NIG#

DGRC (Kyoto)#

  • URL: http://kyotofly.kit.jp/cgi-bin/stocks/index.cgi
  • User ID: 2711
  • Password : dvek7m
  • invoices are sent via email just after flies ship
  • try to pay using the PCard; if you obtain an error message regarding payment just ask Rachel to enter her personal credit card number, or else use your own personal credit card and file for reimbursement
  • importation requires a USDA shipping label and a USDA “letter of no jurisdiction - see below
  • To search by NP number, select Quick Search and enter the full NP designation (e.g., NP3060), or select Advanced Search and enter the number (minus the preceding “NP”, e.g. 3060) in the Original Number area. Be sure select the correct hit (e.g., P{GawB}NP3060).

Importation#

You’ll need to deal with importation paperwork if you’re requesting flies from any source outside the US (whether VDRC, DGRC, or an individual lab outside the US). To import Drosophila melanogaster that harbor only “routine” transgenic constructs, we no longer need a permit. However, we need to supply a “Letter of No Permit Required” (LONPR) from the USDA. Please email thie letter to the sender/vendor so they can print it out and include it with the shipment.

📎 Wilson_LONPR.pdf

For “non-routine” transgenic constructs, the LONPR would not be appropriate: here we would need to apply for a permit from the USDA to import. Non-routine transgenic constructs are defined as follows:

  • Strains carrying transgenic microorganisms causing plant diseases or used for biocontrol of plant pests
  • Strains with select agent sequences. According to the BDSC, no flies have been documented that would fit into this category.
  • Strains where the introduced genetic material is designed to propagate through a population by biasing the inheritance rate. These are generally strains related to gene drive experiments.

Our lab has never used one of these non-routine transgenic constructs, but if you are considering using something like this, please talk to Rachel

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