Pipettes start life as glass tubes that need to be pulled by our micropipette puller so that they taper to a fine point.
Major glass tubes in use#
| item | specs | uses | useful puller programs |
|---|---|---|---|
| Sutter Instrument BF150-110-7.5 | ‘Thinwall’ Heavy Polish Borosilicate glass 1.5 OD 1.1 ID 7.5 cm Long filament: yes | electrophysiology, mostly ‘cleaning’ use instead of un-polished WPI glass to avoid re-chloriding wire after scraping | |
| Sutter Instrument BF150-86-7.5HP | ‘Thickwall’ Heavy Polish Borosilicate glass 1.5 mm OD 0.86 mm ID 7.5 cm Long filament: yes | electrophysiology | |
| Warner G100-F | Pressurised ATP delivery Pressurised KCl delivery | 56 [ATP delivery - Alex Bates] |
Filling a pipette#
You will need to fill your pipette with fluid. To do this:
Equipment:#
- Your pulled glass pipette
- VWR gel-loading tips, cat#76321-828
- Other gel-loading tips may be preferable depending on what you are doing.
- Typicall you want 200ul, ~0.5 mm rounded end tips.
- A Pipetman mechanical pipette or similar
- Your fluid, e.g. saline
Process#
- Load tip into mechanical pipette.
- Load fluid into tip.
- Eject into the back (non-tapered end) of your glass pipette.