This page is meant to keep the current configurations of all the 2-photon microscopes we have in an easy to access way. Information useful for maintenance or troubleshooting, like serial numbers, who used them in the past, RMA numbers, and when components were modified. Try to maintain all sections as you make changes, add in notes if something was repaired or is not functioning properly. Try not to add sections that are under flux unless necessary.

Microscopes are named Berg I through IV, they are all off-the-shelf from Thorlabs and run ScanImage Premium (see /two-photon-imaging/scanimage-setup-download-installation/).

Berg I#

Summary: Located at the ‘back’ of the longer air table with the side-facing light-enclosure opening. It is a galvo-res only system using a tunable laser for imaging and upgraded PMTs. It has NIDAQ-based acquisition capable of 2-channel acquisition.

General#

Model: Thorlabs Bergamo-II

Install Date: 2015

Serial Number: TP01170808-1760

HMS Asset Tag: HMS52040853

Software#

ScanImage Version: >=2023.1.0

ScanImage License: ANQA1-P2Z00-G1HJK-M8P16-582BS-RDPBQG7E3

ScanImage System ID: BERG #1 (MBF System 4) - Rachel Wilson Lab

Hardware#

Imaging Scanhead + Driver: Thorlabs Galvo-Res and Thorlabs ECU

  • This was replaced using the Galvo-Res previously on Berg-II after notable damage to the original mirrors was found. Scanhead driver is NOT matched exactly to the mirrors, but it has not caused any issues so far.
  • This is an older model that does not have galvo (mirror) position feedback, so you cannot calibrate the slow mirror, or optimize the scanning waveforms.
  • The serial for this is the same as the system: TP0117080-1760

Imaging Laser + Modulation: Coherent Vision-S with Conoptics 350-80LA pockels cell

  • This table has a single tunable femtosecond laser split equally between both microscopes
  • Modulation by Conoptics pockels cell within a thorlabs BCM-PCA enclosure
  • Feedback from a photodiode monitoring Pockels cell output sent to ScanImage
  • Pockels Cell Amplifier Model 302RM is on the shelf above the air table, Serial:
  • Note: Conoptics amplifier SN 3605 was repaired in 2018 (not yet sure if is on Berg I or II)

Fast-Z: Physik Instrumente PiFoc Piezoelectric Z-scanner + Piezo Driver

  • Z-scanner part number: P-725K085, serial: 115066019
  • Piezo driver part number: E-709.CHG, serial: 11567108
  • HMS Asseet tag: HMS52041550
  • Feedback about position from the piezoelectric actuator is sent to scanimage, so waveforms for z-stacks can be optimized.

Objective: 20X Olympus XLUMPLFLN Objective, 1.00 NA, 2.0 mm WD

  • HMS asset tag: HMS36462
  • Serial:

Detection: Thorlabs PMT2100 GaAsP PMTs

  • These were upgraded in 2023 (courtesy of Pablo) to the Thorlabs USB-controled/powered models
  • Originally had Hamamatsu H10770PA-40 with Thorlabs transimpedance amplifier TIA60

Acquisition: National Instruments NIDAQ PXIe-1073 Chassis System with cards

  • PMT signals digitized (acquired) on NI 5732

Stage/Objective XY/Z Motor:

  • Unclear if it is the original from install or if was repaired in the years since.

Misc Info#

Users#

  • Pablo
  • Carl

Berg II#

Summary: Located at the ‘front’ of the longer air table with the end-facing light-enclosure opening. It is a galvo-galvo-res system with tunable laser input and an older version of scanimage / PMTs. It has NIDAQ-based acquisition capable of 2-channel acquisition.

General#

Model: Thorlabs Bergamo II

Install Date: 2018

Serial Number: TP01707384-683785

HHMI Asset Tag: 311387

Software#

ScanImage Version: >=2023.1.0

ScanImage License: ANQR1-F2D00-G1HJK-M8P1A-5U2R8-9QDG8CNUB

ScanImage System ID: BERG #2 (MBF System 2) - Rachel Wilson Lab

Multiplicity License: C2A61-4145B-71098-B32F8-1F6D8-CCEDA (Email: stephen_holtz@hms.harvard.edu)

  • Note you need to set an encryption key when this is set up that matches on each computer, it is currently “flypokers” no quotes.

Hardware#

Imaging Scanhead + Driver: Vidrio Technologies (now MBF) RMR

  • Scanner+Driver S/N: RMR18721A
  • This was swapped out in 2022, upgraded from a thorlabs galvo-res scanner

Imaging Laser + Modulation: Coherent Vision-S with Conoptics 350-80LA pockels cell

  • This table has a single tunable laser split equally between both microscopes
  • Modulation by Conoptics pockels cell within a thorlabs BCM-PCA enclosure
  • Feedback from a photodiode monitoring Pockels cell output sent to ScanImage
  • Pockels Cell Amplifier Model 302RM is on the shelf above the air table, serial:
  • Note: Conoptics amplifier SN 3605 was repaired in 2018 (not yet sure if is on Berg I or II)

Fast-Z: Physik Instrumente PiFoc Piezoelectric Z-scanner + Piezo Driver

  • Z-scanner part number: P-725K085, serial: 118050375
  • Driver part number: E-709.CHG, serial: 118052677

Objective: 20X Olympus XLUMPLFLN Objective, 0.95 NA

  • HMS asset tag: HMS35769
  • Serial:
  • (Will be 20X Olympus XLUMPLFLN Objective, 1.00 NA, 2.0 mm WD)

Detection: Hamamatsu H10770PA-40 with Thorlabs transimpedance amplifier TIA60

  • Original to installation (as far as we can tell)
  • Planned upgrade to this in 04/2024 to Thorlabs PMT2100

Acquisition: National Instruments NIDAQ PXIe-1073 Chassis System with cards

  • PMT signals digitized (acquired) on NI 5732

Stage/Objective XY/Z Motor:

  • Repaired/replaced stage motors in 2021 after a spill.

Misc Info#

Users#

  • Sophia
  • Jingxuan
  • Yunzhi
  • Helen

Berg III#

Summary: Located at the ‘rear’ of the table with the box opening facing the room wall. It is a dual laser line system with galvo-galvo-res system with tunable laser and/fixed wavelength imaging input, and galvo-galvo fixed wavelength input. It has a standard vDAQ which can be expanded to 4 PMT channels.

General#

Model: Thorlabs Bergamo II with RGG and GG paths.

Install Date: 2021

Serial Number: TP02569780-6888827

HHMI Asset Tag: BA100002387

Software#

ScanImage Version: 2023.1.0

ScanImage License: ANQA1-F2200-G1HJK-M8P12-552YT-KMVURHV4M

ScanImage System ID: BERG #3 (MBF System 1) - Rachel Wilson Lab

Multiplicity: BD824-C4CDF-0C2C5-FBDE0-06B4C-28DAA (Email: stephen_holtz@hms.harvard.edu)

Hardware#

Imaging Scanhead: + Driver: Thorlabs resonant-galvo-galvo (RGG) and LSKRGG

  • One of the ‘red boxes’ on the rack
  • Scanhead Serial:
  • Driver Serial: NONE MARKED, installed at the same time as the Berg IV system which has serial TP03239622-692598

Imaging Laser + Modulation: Mai Tai HP 1040S (with DeepSee) and Conoptics 350-80LA

  • This table has a single tunable laser split equally between both microscopes
  • Modulation by Conoptics pockels cell within a thorlabs BCM-PCA enclosure
  • Feedback from a photodiode monitoring Pockels cell output sent to ScanImage
  • Pockels Cell Amplifier Model 302RM (on the shelf above the air table) Serial: 4693, likely repaired in 2015

Stimulation Laser + Modulation: Axon 1064-3 TPC

  • This laser is split evenly between Berg III and IV and is modulated by BNC input, it must be switched between the two manually with a BNC switch.
  • Scanhead Serial:
  • Driver Serial: GDP.1007S.7830, Date: 29.09.2023
  • HHMI Asset Tag: BA100004029

Stimulation Scanhead + Driver: Thorlabs galvo-galvo and LSKGG4/M

  • Driver is one of the ‘red boxes’ on the rack
  • Scanhead serial:
  • Driver Serial: TP03239622-692596, MFD 2023

Fast-Z: Thorlabs PFM450 Piezo Objective Scanner + Controller

  • Serial: 44204334 (2021)

Objective: 25X Nikon CFI APO LWD 1.10 NA, 2.0 mm WD

  • Serial:
  • HHMI Asset Tag:

Detection: Thorlabs PMT2001 PMTs x2

  • USB powered/controlled packaged PMTs
  • Original to installation as of 03/2024

Acquisition: Vidrio (MBF) vDAQ

  • 4-channel version of the vDAQ

Stage/Objective XY/Z Motor: Thorlabs

  • Original to installation

Misc Info#

  • Switching between stimulation + imaging versus 2-color imaging requires replacing a mirror above the objective within the microscope. It may also require adjusting the split ratio of the fixed wavelength laser input between imaging and stimulation paths.

Users#

  • Alex
  • Wenyi
  • Carl
  • Yunzhi

Berg IV#

Summary: Located at the ‘front’ of the table with the box opening facing the laser safety curtain. It is a dual laser line system with galvo-galvo-res system with tunable laser and/fixed wavelength imaging input, and galvo-galvo fixed wavelength input. It also has a high-NA objective with a high-speed vDAQ.

General#

Model: Thorlabs Bergamo II with RGG and GG paths.

Install Date: 2021

Serial Number: TP02577043-688848

HHMI Asset Tag: BA100002388

Software#

ScanImage Version: 2023.1.0

ScanImage License: ANQE1-Y2X00-G1HJK-M8P1W-5J2A3-5GJ9IF8ZU

ScanImage System ID: BERG #4 (MBF System 3 ) - Rachel Wilson Lab

Hardware#

Imaging Scanhead + Driver: Thorlabs resonant-galvo-galvo (RGG) and LSKRGG

  • Driver is one of the ‘red boxes’ on the rack
  • Scanhead serial:
  • Driver Part Number: 30-0994 / Serial: TP02577043-688848

Imaging Laser + Modulation: MaiTai-DeepSee with Conoptics 350-80LA pockels cell

  • This table has a single tunable laser split equally between both microscopes
  • Modulation by Conoptics pockels cell within a thorlabs BCM-PCA enclosure
  • Feedback from a photodiode monitoring Pockels cell output sent to ScanImage
  • Pockels Cell Amplifier Model 302RM (on the shelf above the air table) Serial: 3868, repaired in 2018

Stimulation Laser + Modulation: Axon 1064-3 TPC

  • This laser is split evenly between Berg III and IV and is modulated by BNC input, it must be switched between the two manually with a BNC switch.
  • Scanhead serial:
  • Driver Serial: GDP.1007S.7830, Date: 29.09.2023
  • HHMI Asset Tag: BA100004029

Stimulation Scanhead + Driver: Thorlabs galvo-galvo and LSKGG4/M

  • Driver is one of the ‘red boxes’ on the rack
  • Scanhead serial:
  • Driver Serial: TP03239622-692598, MFD 2023

Fast-Z: Thorlabs PFM450 Piezo Objective Scanner + Controller

  • Serial: 44862608 (2016)

Objective: 25X Nikon CFI APO LWD 1.10 NA, 2.0 mm WD

  • HHMI Asset Tag: 311192
  • Serial: MRD77220

Detection: Thorlabs PMT2001 PMTs x2

  • USB powered/controlled packaged PMTs
  • Original to installation as of 03/2024

Acquisition: Vidrio (MBF) High-Speed vDAQ

  • 2 channel ‘high-speed’ version of the vDAQ that is compatible with photon-counting

Stage/Objective XY/Z Motor: Thorlabs

  • Original to installation

Misc Info#

  • Switching between stimulation + imaging versus 2-color imaging requires replacing a mirror above the objective within the microscope. It may also require adjusting the split ratio of the fixed wavelength laser input between imaging and stimulation paths.

Users#

  • Diego (current)
  • Stephen (current)
  • Noah (current)
  • Parisa

Misc Information#